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KMID : 0861020190340010099
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Korea Journal of Herbology
2019 Volume.34 No. 1 p.99 ~ p.107
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Investigation of the effect of water extract from Cudrania tricuspidata fruit on tight junction in human keratinocyte
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Lee Sang-Soo
Choi Sun-Kyung
Kim Jae-Whan
Han Hyo-Sang
Kim Kee-Kwang
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Abstract
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Objectives : Cudrania tricuspidata (C. tricuspidata) is well-known traditional herbal remedy and its root, leaf and fruit were used for treatment of inflammation, tumor and painkilling. However, effect of C. tricuspidata fruit on tight junction is still unknown. The aim of this research was to determine the effect of C. tricuspidata fruit extract on human keratinocyte HaCaT cells.
Methods : The antioxidant effects of water extract of C. tricuspidata (WECT) and ethanol extract of C. tricuspidata (EECT) were analyzed by using an ABTS assay. To confirm the cytotoxicity of WECT and EECT, MTS assay was performed. The mRNA expression levels of tight junction related genes were analyzed using quantitative RT-PCR analysis. Furthermore, dispase assay was used to investigate the alteration of cell?cell adhesion strength of EECT treated HaCaT cells.
Results : WECT and EECT showed strong antioxidant activity. No obvious cytotoxicity was observed in both WECT and EECT until 2.0 ㎎/㎖ concentration. The mRNA expression level of Claudin 6 were significantly increased by EECT treatment, whereas the WECT did not affect the expression of Claudin 6. Furthermore, EECT treatment enhances cell-cell adhesion strength.
Conclusions : In this study, we investigated the physiological activities of the extracts of Cudrania tricuspidata fruit extracts on human keratinocytes by two different extraction methods. EECT might have an anti-aging activity on the skin by reducing oxidative stress. Moreover, it may be a useful ingredient in atopic dermatitis and skin- moisturizing, given its effects of altering Claudin 6 gene expression and enhancing cell?cell adhesion strength.
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KEYWORD
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Cudrania tricuspidata fruit, Human keratinocyte, Tight junction, Claudin 6
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